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Journal for ImmunoTherapy of Cancer

Open Access

WT1 peptide analogue WT1-126Y enhances leukemia lysis

  • Ghofran Alqudaihi1,
  • Cynthia Lehe2,
  • Monther Al-Alwan1,
  • Hazem Ghebeh1,
  • Anne Dickinson3 and
  • Said Dermime4
Journal for ImmunoTherapy of Cancer20142(Suppl 1):O3

Published: 24 February 2014


PeptideTumour AntigenBinding AbilityLytic ActivityPotential Approach


The Wilms Tumour Antigen 1 (WT1) has been shown to be expressed at low levels in some normal cells. Therefore, many of the potential CTL epitopes against this antigen may be absent or suboptimal. To this end, different groups introduced modifications in the sequence of the anchor positions of these “sub-optimal” peptides to improve their binding to HLA class I molecules and to increase their immunogenicity.


To explore the feasibility of using an approach that enhances the immunogenicity of low-avidity restricted peptides without altering their antigenic specificity. This approach consists of replacing the first amino acid of two known HLA-A0201-restricted WT1-derived peptides (Db126 and WH187) by a tyrosine (Y).


The modified WT1-Db126 showed enhanced binding ability to the HLA-A*0201 molecule, increased the frequency of IFN-γ producing cytotoxic T lymphocyte (CTL) and boosted the lytic activity of the generated CTL against HLA-matched leukaemia cells. Interestingly, the CTL line generated with the modified epitope was able to recognize the wild-type peptide presented by target cells.


This study provide evidence that peptide modification results in a better immune response against cancer and further support the use of this strategy as a potential approach for the development of a leukemia-vaccine.

Authors’ Affiliations

King Faisal Specialist Hospital & Research center, Riyadh, Saudi Arabia
Dasman Diabetes Institute, Kuwait
University of Newcastle upon Tyne, Newcastle Upon Tyne, UK
King Fahad Specialist Hospital, Dammam, Saudi Arabia


© Alqudaihi et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.