Characterization and quantification of tumor infiltrating lymphocytes in breast cancer
© Bedri et al; licensee BioMed Central Ltd. 2014
Published: 24 February 2014
Tumor infiltrating lymphocytes (TILs) are a significant component of the tumor microenvironment . In breast cancer the prognostic and predictive value of TIL is under active investigation . The goal of our study was to define specific TIL subtypes and regional quantification in breast cancer of patients from the MENA region.
47 FFPE samples with known clinic-pathological data were selected and studied using immunohistochemistry technique. TIL immune markers studied were CD3, CD8, CD45RO and FOXP3 and quantified by modified H-score system. Results were analyzed via SPSS.
A positive correlation between CD3 and CD8 expression (rs=0.614, n=24, P 0.001) and CD3 and CD45RO expression (rs=0.621, n=24, P 0.001) were noted in the center of the tumor (CT).CD3 median percentage in CT was higher in grade 2 and grade 3 breast carcinoma compared to grade 1 (P 0.023). CD3 in the center of the tumor and CD8 in the invasive margins (IM) were significantly higher in ER/PR negative Her2/neu positive tumors compared to triple negative breast cancers. Lower CD3, CD8 and FOXP3 percentages were noticed in TNBC tumors. CD45RO median percentage was shown to be higher in the IM region.
TIL were affected by the patient clinic-pathological characteristics.
- Hanahan D, Weinberg RA: Hallmarks of cancer: the next generation. Cell. 2011, 144 (5): 646-74. 10.1016/j.cell.2011.02.013. doi: 10.1016/j.cell.2011.02.013. Review. PubMed PMID: 21376230View ArticlePubMedGoogle Scholar
- Mahmoud SM, Paish EC, Powe DG, Macmillan RD, Grainge MJ, Lee AH, Ellis IO, Green AR: Tumor-infiltrating CD8+ lymphocytes predict clinical outcome in breast cancer. J Clin Oncol. 2011, 29 (15): 1949-55. 10.1200/JCO.2010.30.5037. doi: 10.1200/JCO.2010.30.5037. Epub 2011 Apr 11. PubMed PMID: 21483002View ArticlePubMedGoogle Scholar
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