Volume 3 Supplement 2

30th Annual Meeting and Associated Programs of the Society for Immunotherapy of Cancer (SITC 2015)

Open Access

Safety, feasibility and functionality of activated autologous dendritic cells for intratumoral injection in solid tumors: a Phase I clinical trial

  • Vivek Subbiah1,
  • Ravi Murthy1,
  • David Hong2,
  • Robert Prins3,
  • Chitra Hosing1,
  • Robert Brown4,
  • McGuire Mary4,
  • Aung Naing1,
  • Siquing Fu1,
  • Anthony Conley1,
  • Indreshpaul Kaur1,
  • Kyle Hendricks5,
  • Deepthi Kolli5,
  • Lori Noffsinger6 and
  • Marnix Bosch7
Journal for ImmunoTherapy of Cancer20153(Suppl 2):P199

DOI: 10.1186/2051-1426-3-S2-P199

Published: 4 November 2015

Background

Dendritic cells (DC) are proficient in initiating adaptive immune responses, through the uptake and subsequent presentation to the immune system of antigenic compounds. In preclinical studies, activated DC (aDC; DCVax®-Direct) were shown to be superior to immature DC in clearing tumors from mice, upon intratumoral injection.

Methods

Forty patients were enrolled in a Phase I dose escalation trial to test the safety and feasibility of intratumoral injection of aDC in solid tumors. aDC were administered intratumorally under image guidance, at a dose of 2 million, 6 million, or 15 million live, activated, autologous DC per injection. At each injection visit (days 0, 7, 14, then weeks 8, 16 and 32), a single lesion was injected. Biopsies were assessed for tumor necrosis and for infiltrating lymphocytes. Tumor size was monitored through standard imaging procedures, and blood was collected for immune monitoring. The aDC were assessed for expression of co-stimulatory molecules and for the production of cytokines.

Results

Intratumoral injection under image guidance was generally well tolerated and feasible. In total, 149 i.t. injections were performed, in 17 patients at the 2 million, 20 at the 6 million, and 3 at the 15 million dose level, with mild to moderate fevers as the most frequently observed adverse events. Biopsies of the injected tumors showed appearance of tumor necrosis in 62%, and T cell infiltrates in 54%. Stabilization of disease was found to correlate with survival, and with a specific cytokine profile of the aDC which is consistent with induction of Th-1 type immune responses.

Conclusions

Intratumoral injection of autologous, activated DC is feasible without significant toxicity in multiple solid tumors, and can elicit local and systemic immune responses. Specific characteristics of the injected dendritic cells may predict tumor response and survival.

Trial registration

ClinicalTrials.gov identifier NCT01882946.

Authors’ Affiliations

(1)
U.T.M.D. Anderson Cancer Center
(2)
The University of Texas MD Anderson Cancer Center
(3)
UCLA Neurosurgery
(4)
UT Health, University of Texas Health Science center at Houston
(5)
Cognate Bioservices, Inc
(6)
Cognate Bioservices, Inc
(7)
Northwest Biotherapeutics

Copyright

© Subbiah et al. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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