Volume 3 Supplement 2

30th Annual Meeting and Associated Programs of the Society for Immunotherapy of Cancer (SITC 2015)

Open Access

Identification of tumor-specific T cell receptors from primary tumor-infiltrating lymphocytes

  • Hiroyuki Kishi1,
  • Hiroshi Hamana1,
  • Kiyomi Shitaoka1 and
  • Atsushi Muraguchi1
Journal for ImmunoTherapy of Cancer20153(Suppl 2):P74

DOI: 10.1186/2051-1426-3-S2-P74

Published: 4 November 2015

Antigen-specific adaptive T cell therapy or T cell receptor (TCR) gene therapy is promising as a next generation tumor therapy. For the therapy, the production of tumor-specific T cells or their TCRs is prerequisite. Exploration of tumor-associated antigens, antigen-specific T cells or their TCR is usually restricted to the population with major HLA haplotypes, such as HLA-A24 for the Southeast Asian or HLA-A2 for Caucasian population. In this context, antigen-specific T cell therapy or TCR gene therapy is restrictive to those with major HLA haplotypes but is not beneficial for those with minor HLA haplotypes. In this study, we analyzed the TCRαβ repertoire of tumor-infiltrating lymphocytes (TIL) of B16F10 melanoma in C57BL/6 mice at single cell levels. We found the clonal expansion of T cells in TILs, but not in splenic T cells and detected tumor-specific T cells in those clonally expanded cells. Our single cell analysis system may greatly contribute to analyze tumor-specific T cells without restriction in HLA haplotypes, thus leading to personalized tumor immunotherapy in the future.

Authors’ Affiliations

(1)
University of Toyama

Copyright

© Kishi et al. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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