Concurrent SPECT/PET-CT imaging as a method for tracking adoptively transferred T-cells in vivo
© Stanton et al. 2016
Received: 11 February 2016
Accepted: 15 April 2016
Published: 17 May 2016
The ability of T-cells to traffic to and penetrate tumors impacts the clinical efficacy of T-cell therapy therefore methods to track transferred T-cells in vivo are needed. In this preliminary report, we evaluated the use of concurrent SPECT/PET-CT imaging to monitor the egress of HER-2/neu specific T-cells in a breast cancer patient with extensive bone-only metastatic disease.
Indium (In-111) labeled T-cells demonstrated similar or greater viability than unlabeled T-cells at either a low or high dose of In-111 over a 24-h incubation period in vitro. The function of labeled or unlabeled T-cells was not significantly different (p > 0.05) at either dose. T-cells trafficked to all sites of metastatic disease and infiltrated the tumor as assessed by SPECT imaging. In-111 uptake at 24 h after infusion varied from 3.8 (right proximal humerus) to 6.3 (right sacrum) background corrected counts per pixel and remained elevated at 48 h. Concurrent PET-CT imaging demonstrated a fluorodeoxyglucose flare, measured by increase in tumor site uptake as high as 32 % and at most sites of disease at 48 h. This flare was associated with focal pain after T-cell infusion at metastatic sites. The patient had stable disease for 18 months after completion of T-cell therapy.
Concurrent SPECT/PET-CT imaging, over a 48-h period after T-cell infusion, provided evidence of T-cell homing to all disease sites as well as a tumor metabolism flare response. This technique may be useful for monitoring T-cell trafficking after autologous as well as chimeric antigen receptor T-cell infusion.
Trial registered at ClinicalTrials.gov registration number NCT00791037, registered 13 November 2008.
KeywordsAdoptive T-cell therapy HER2 breast cancer Indium-111 labeled FDG PET-CT SPECT
Adoptive T-cell therapy using chimeric antigen receptor, tumor infiltrating, or antigen specific T-cells shows significant clinical responses in hematologic cancers but few durable responses in solid tumors [1–3].Limitations include the inability of antigen specific T-cells to traffic to and penetrate solid tumors and significant variations in tumor T-cell infiltration . For example, in adoptive transfer of HER2 specific T-cells, indium 111 (In-111) labeled cells could traffic to the bone marrow but not to liver metastases . Methods are needed to monitor infused T-cells in vivo, evaluate their function, and demonstrate whether T-cells penetrate all metastatic sites.
Readily accessible approaches for monitoring T cells in vivo are tracking T-cells labeled with In-111 by single photon emission computed tomography (SPECT) imaging or evaluating T-cell activity by fluorodeoxyglucose positron emission tomography (PET-CT) . As part of a clinical trial, we piloted the concurrent use of SPECT and PET-CT imaging to assess the homing capability and inflammatory function of adoptively transferred antigen specific T-cells at various metastatic sites.
Indium-111 labeling does not impact T-cell viability nor diminish antigen specific cytokine secretory function
In-111 labeling did not diminish the ability of T-cells to secrete IFN-gamma (IFN-g). When cultured with IL-2, 49uCi labeled T-cells secreted 336.0 ± 25.4 pg/mL (p = 0.09) and 480uCi labeled T-cells 194.5 ± 16.3 pg/mL (p = 0.07) IFN-g as compared to unlabeled T-cells. 49uCi labeled T-cells 1215.0 ± 8.5 pg/mL (p = 0.03) and 480uCi labeled T-cells 1139.0 ± 46.7 pg/mL (p = 0.02) secreted more IFN-g in response to CMV antigen than unlabeled cells. All groups responded equally to stimulation with CD3/CD28 beads (p < 0.05 for all groups) (Fig. 1b).
HER2 specific T-cells trafficked to and infiltrated all sites of metastatic disease by 48 h
FDG PET-CT demonstrated acute increases in tumor metabolism at most metastatic sites 48 h after T-cell infusion
Location of FDG tumor uptake mirrored the patient’s report of grade 1 or 2 bone pain flares localized to sites of metastatic disease experienced with successive T-cell infusions. After completion of T-cell infusions, the patient had similar boney pain during HER2 specific booster vaccinations. On completion of the study, the patient experienced stable disease without changes in therapy for 18 months.
The use of SPECT imaging is an effective modality for tracking T-cells in vivo without impacting their function. Early trials have demonstrated that Melan-A-specific cytotoxic T-cells labeled with In-111 can traffic to sites of metastasis by 48 h and remain up to 14 days . We demonstrate T-cells homing to multiple sites of breast cancer deposits through In-111 labeling.
Changes in FDG tumor uptake quantitated by PET-CT are a functional measure usually associated with disease progression. In 28 breast cancer patients with bone dominant disease, higher tumor SUV predicted a shorter time to disease progression (p < 0.006) . In our patient, an acute increase in tumor FDG uptake was associated with disease stabilization. After adoptive T-cell therapy for nasopharyngeal carcinoma or breast cancer, pain at sites of disease temporally related to T-cell infusion correlated with clinical response in specific lesions [3, 10]. Acute increases in tumor metabolism by FDG PET-CT allows quantitation of inflammation induced by activated T-cells and may provide a measure of the level needed for disease regression. More data is needed to calculate a correlation of clinical response with specific changes in SUV.
Optimizing adoptive T-cell therapy for all patients requires an understanding of the trafficking and function of these cells in vivo. This pilot data demonstrates that concurrent In-111 T-cell SPECT and FDG PET-CT imaging, modalities available at any US medical center, can evaluate T-cell homing, tumor penetration by T-cells, and T-cell induced inflammation. These preliminary findings will be expanded in further trials.
Materials and methods
T-cell viability and cytokine secretion after In-111 labeling
Peripheral blood mononuclear cells (PBMC) were obtained by apheresis from CMV positive donors. T-cells were cultured with CD3/CD28 beads (Life Technologies), diluted 0.6-1x106/ml, and cultured with IL-2 (30U/ml) every 3 days. Day 12, CD3/CD28 beads were removed and expanded T-cells, >90 % CD3+, were labelled with 48uCi or 490uCi In-111 at GE Healthcare (Seattle, WA). 2 mCi In-111 oxine (Covidien) was added to 200x106 cultured T-cells for 30 min and resuspended in media with non-labelled cells as controls. Cells (1x106/ml per group) were cultured at 37 °C with (1) media only, (2) IL-2 (30U/ml), (3) CD3/CD28 beads (2:1 ratio), or (4) CMV lysate (2.5ug/ml) with irradiated autologous PBMC. 100ul cell suspension was removed at 4 and 24 h. Numbers of live and dead cells were counted in duplicate using trypan blue exclusion and percent viability calculated. Supernatants were collected from the cultured cells 48 h after stimulation and IFN-g release was measured using ELISA (eBioscience).
After informed consent, the patient was enrolled in IRB approved trial NCT00791037. Imaging protocols were approved by IRB and radiation safety. Enrollment criteria included (1) metastatic treatment refractory HER2+ cancer, (2) lesions quantifiable by imaging, and (3) no cytotoxic chemotherapy for 1 month before the first T-cell infusion. The patient was vaccinated with three HER2 specific peptide-based vaccines weekly for 3 weeks . Two weeks after the last vaccine, PBMC were collected by leukapheresis and expanded ex vivo as previously described . Cells were administered as a dose escalation with 5x109 (dose 1), 5x1010 (dose 2), and 5x1011 (dose 3) total cells.
Indium labeling and SPECT imaging
1 mCi In-111 oxine (Covidien) was added to 10x106 HER2 antigen cultured T-cells for 30 min at room temperature and resuspended in normal saline (GE Healthcare Nuclear Pharmacy). Labeled cells were infused prior to the third T-cell infusion. Migration of T-cells was evaluated at 4, 24 and 48 h by whole body planar and SPECT scintigraphy gamma camera imaging (Phillips Precedence; GE Medical Systems) equipped with medium energy collimators. In-111 lesion uptake was quantified by region of interest (ROI) in each metastasis with background subtracted from adjacent soft tissues in counts/pixel. At each time point an aliquot of the injectate was also imaged to calculate radioactive decay for image analysis. Data is reported as counts/pixel normalized to background (ccpp).
The patient was injected with an activity of 310 MBq (10 mCi) of fluorodeoxyglucose (FDG). Imaging was performed by PET-CT scanner (Discovery LS; GE Medical Systems) in high sensitivity mode and consisted of a torso survey covering 5 adjacent 15-cm axial fields. Baseline FDG PET-CT scan was performed in parallel with SPECT imaging prior to infusion and at 48 h. FDG PET-CT was also performed 3 months after the last T-cell infusion. The FDG uptake in metastases was measured as the tumor ROI compared to an equivalent background normalized by injected dose (SUV). Change in FDG uptake early after T-cell infusion was reported as the ratio of FDG SUV per metastatic site increase compared to baseline.
Ethics Approval and Consent to Participate/Publish
The human subject research included in this manuscript was performed in accordance with the declaration of Helsinki and the IRB of record for this study is Fred Hutchinson Cancer Research Center/University of Washington Consortium IRB. This review includes a Scientific Review Committee and IRB review of original application, modifications and annual renewals. IRB Reference Number: IR# 6658. Written consent included consent for publication.
single photon emission computed tomography
fluorodeoxyglucose positron emission tomography
standardized uptake value
corrected counts per pixel
M.L. Disis was supported by R01 CA129517, CA136632, the Athena Distinguished Professor of Breast Cancer Research award, and a Komen Leadership award. S.E. Stanton was supported by NIH KL2TR000421. Clinical work was conducted through the Clinical Research Center Facility at the University of Washington (NIH grant UL1TR000423).
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- Porter DL, Hwang WT, Frey NV, Lacey SF, Shaw PA, Loren AW, et al. Chimeric antigen receptor T cells persist and induce sustained remissions in relapsed refractory chronic lymphocytic leukemia. Sci Transl Med. 2015;7(303):303ra139. doi:10.1126/scitranslmed.aac5415.View ArticlePubMedGoogle Scholar
- Rosenberg SA, Yang JC, Sherry RM, Kammula US, Hughes MS, Phan GQ, et al. Durable complete responses in heavily pretreated patients with metastatic melanoma using T-cell transfer immunotherapy. Clin Cancer Res. 2011;17(13):4550–7. doi:10.1158/1078-0432.CCR-11-0116.View ArticlePubMedPubMed CentralGoogle Scholar
- Disis ML, Dang Y, Coveler AL, Marzbani E, Kou ZC, Childs JS, et al. HER-2/neu vaccine-primed autologous T-cell infusions for the treatment of advanced stage HER-2/neu expressing cancers. Cancer Immunol Immunother. 2014;63(2):101–9. doi:10.1007/s00262-013-1489-4.View ArticlePubMedPubMed CentralGoogle Scholar
- Abastado JP. The next challenge in cancer immunotherapy: controlling T-cell traffic to the tumor. Cancer Res. 2012;72(9):2159–61. doi:10.1158/0008-5472.CAN-11-3538.View ArticlePubMedGoogle Scholar
- Bernhard H, Neudorfer J, Gebhard K, Conrad H, Hermann C, Nahrig J, et al. Adoptive transfer of autologous, HER2-specific, cytotoxic T lymphocytes for the treatment of HER2-overexpressing breast cancer. Cancer Immunol Immunother. 2008;57(2):271–80. doi:10.1007/s00262-007-0355-7.View ArticlePubMedGoogle Scholar
- Schauwecker DS, Burt RW, Park HM, Mock BH, Tobolski MM, Yu PL, et al. Comparison of purified indium-111 granulocytes and indium-111 mixed leukocytes for imaging of infections. J Nucl Med. 1988;29(1):23–5.PubMedGoogle Scholar
- Aarntzen EH, Srinivas M, Bonetto F, Cruz LJ, Verdijk P, Schreibelt G, et al. Targeting of 111In-labeled dendritic cell human vaccines improved by reducing number of cells. Clin Cancer . 2013;19(6):1525–33. doi:10.1158/1078-0432.CCR-12-1879.View ArticleGoogle Scholar
- Meidenbauer N, Marienhagen J, Laumer M, Vogl S, Heymann J, Andreesen R, et al. Survival and tumor localization of adoptively transferred Melan-A-specific T cells in melanoma patients. J Immunol. 2003;170(4):2161–9.View ArticlePubMedGoogle Scholar
- Specht JM, Tam SL, Kurland BF, Gralow JR, Livingston RB, Linden HM, et al. Serial 2-[18 F] fluoro-2-deoxy-D-glucose positron emission tomography (FDG-PET) to monitor treatment of bone-dominant metastatic breast cancer predicts time to progression (TTP). Breast Cancer Res Treat. 2007;105(1):87–94. doi:10.1007/s10549-006-9435-1.View ArticlePubMedGoogle Scholar
- Comoli P, Pedrazzoli P, Maccario R, Basso S, Carminati O, Labirio M, et al. Cell therapy of stage IV nasopharyngeal carcinoma with autologous Epstein-Barr virus-targeted cytotoxic T lymphocytes. J Clin Oncol. 2005;23(35):8942–9. doi:10.1200/JCO.2005.02.6195.View ArticlePubMedGoogle Scholar
- Disis ML, Gooley TA, Rinn K, Davis D, Piepkorn M, Cheever MA, et al. Generation of T-cell immunity to the HER-2/neu protein after active immunization with HER-2/neu peptide-based vaccines. J Clin Oncol. 2002;20(11):2624–32.View ArticlePubMedGoogle Scholar