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Comparative immunohistochemical analysis of Beclin-1 & MDM-2 in benign & malignant ameloblastomas
- MA Elbarrawy1
© Elbarrawy 2015
Published: 4 November 2015
Ameloblastoma is the most frequently encountered neoplasm arising from the odontogenic epithelium. Beclin-1 protein plays a critical role in autophagy as a tumor suppressor gene. Whereas, the Murine Double Minute 2 (MDM-2) is a cellular proto-oncogene capable, if amplified, of causing tumor-genesis. The expression & prognostic significance of both genes are largely unexplored, yet, in this neoplasia. Therefore, the present investigation aimed to assess their possible biological role in ameloblastomas.
This study was done among 35 studied cases: 29 cases of benign ameloblastomas, and 6 cases of ameloblastic carcinomas. Labeled Streptavidin Biotin (LSAB + Dako) immunohistochemical method, utilizing monoclonal antibodies for Beclin-1 & MDM-2 genes, was used.
Most of the benign ameloblastomas, 25 out of 29 cases (86%), showed intense total cell positivity for the Beclin-1, while, the ameloblastic carcinomas revealed mild (3 out of 6 cases, 50%) to negative expression (3 cases: 50 %). Inversely, the MDM-2 oncoprotein demonstrated intense brown total cell reactivity in amelobastic carcinoma (5 out of 6 cases, 83% positivity) & loss of the reaction (21 cases: 72%) to mild brown stain (8 cases:28%) in benign ameloblastoma. These findings were statistically significant.
Based from these findings, one could conclude that, MDM-2 could be a specific marker to identify the proliferative activity, tumor aggressiveness & directly proportional with the degree of malignancy. In contrast, the high Beclin-1 expression could be a good indicator of prognosis in ameloblastomas. Hence, an overall comparison, both studied genes may be very promising molecular prognostic biomarkers.
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.