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Immunotherapy with patient-specific antigens selection reduced the metastasis of a cervical cancer patient
© Han et al. 2015
Published: 4 November 2015
Cervical cancer is the second most common gynecologic malignant tumor, and is frequently associated with human papilloma virus (HPV) infection. Patients with vascular invaded tumors are more likely to develop metastatic disease after radical resection of primary tumor. Current treatment for metastatic cervical cancer is not effective. In 2011, a patient was diagnosed as HPV+ cervical squamous cell carcinoma with vascular invasion. 33 months after radical resection, and subsequent adjuvant chemoradiation therapy, metastasis was detected on the right sacroiliac joint. Given that standard therapy was unsuccessful, the patient was treated with MASCT (Multiple Antigen Stimulating Cellular Therapy), which is composed of multiple tumor antigen pulsed dendritic cells (DCs) and autologous T lymphocytes activated by these DCs.
Monocytes from the patient's PBMCs were differentiated into immature dendritic cells (iDCs) and then pulsed with multiple synthetic peptide antigens including tumor-associated antigens and HPV specific antigens. The semi-mature DCs were further stimulated by diverse TLR ligands to differentiate into mature DCs (mDCs). Half of these mDCs were subcutaneous injected to the patient, and the other half were co-cultured with the maintaining non-adherent T cells for another 7-9 days before infusion back to the patient.
Our study provides MASCT with as a safe treatment, which has reduced the metastasis of the cervical cancer patient. Moreover, tumor antigens specific T cell responses could be robustly raised in cervical cancer patients after MASCT treatment, and were even further boosted after patient-specific antigens selection.
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.