Skip to content

Advertisement

  • Poster presentation
  • Open Access

Tetramer based approach for efficient identification and isolation of neo-antigen specific CD8 T cells from peripheral blood (PBL) of patients with metastatic cancers

  • 1,
  • 2,
  • 2,
  • 1,
  • 2,
  • 2,
  • 3,
  • 1 and
  • 3
Journal for ImmunoTherapy of Cancer20153 (Suppl 2) :P47

https://doi.org/10.1186/2051-1426-3-S2-P47

  • Published:

Keywords

  • Metastatic Cancer
  • Exome Sequencing
  • Mutate Amino Acid
  • Adoptive Cell Therapy
  • Autologous CD14

Background

Adoptive cell therapy with T cells bearing mutation specific T cell receptors (TCR) can be an effective method for treating metastatic cancers. The objective of this study was to identify mutation reactive T cells in the circulation of patients with different types of metastatic cancer.

Methods

The strategy utilized whole exome sequencing data to identify somatic non-synonymous mutations and then in-silico algorithms to predict minimal epitopes encoding mutated amino acids for each patient specific HLA-allele. CD8-enriched PBL from each patient were stained with tetramers generated in house by a UV-exchangeable technique as previously described for A*02:01, A*03:01, A*11:01, B*07:02, and a commercial tetramer was acquired for B*57:01. Based on the initial staining frequency (+tetramer+ T cells recognizing 7 unique neo-antigens from the PBL of 4 patients (ranging from 1 to 4 per patient). We enriched the frequencies of CD8+tetramer+ cells from 0.5 to >85%, 0.3 to >65% and 0.01 to 3% from the PBL of patients with colorectal (3971-A*02:01), lung (4014-B*57:01), and ovarian (4067-B*07:02) cancers respectively, using individual tetramers. Populations reactive with three HLA-A*11:0-restricted and one HLA-A*03:01-restricted neoantigens were also isolated from the PBL of lung cancer patients 4014 and 4037, respectively, using a pooled tetramer approach.

Results

Overall, the isolated T cells recognized mutated epitopes when co-cultured with autologous CD14+ monocytes pulsed with mutated peptides in the context of appropriate MHC-I alleles including HLA-A*02:01, HLA-A*03:01, HLA-A*11:01, HLA-B*07:02, and HLA-B*57:01, with reactivity detected using IFN-γ ELISA. Using single cell PCR, we could clone the TCRs reactive with an HLA-*02:01-presented colon cancer neoantigen and an HLA-B*57:01-presented lung cancer neoantigen. Evaluation of PBL retrovirally-transduced with these TCRs demonstrated that they bound to tetramers and secreted IFN-γ when co-cultured with CD14+ monocytes pulsed with appropriate mutated peptides.

Conclusions

To conclude, tetramers offer a sensitive, fast and reliable methodology to isolate mutation specific tumor reactive T cells from PBL of cancer patients. Furthermore, this method facilitates the identification, and cloning of mutation reactive TCR with which to construct receptor-engineered T cells for adoptive T cell therapy.

Authors’ Affiliations

(1)
NIH/NCI, Bethesda, MD, USA
(2)
NIH/NCI Surgery Branch, Bethesda, MD, USA
(3)
National Institutes of Health, Bethesda, MD, USA

Copyright

© Bharathan et al. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Advertisement