Serum levels of cytokine upon combined CD1d treatment and OVA/CpG immunization. Mice were immunised i.m. with OVA peptide alone or in combination with CpG or CD1d-fus/CpG following Vα14-Jα18 and OT-I cell transfer and treated or not systemically with CD1d-fus. Sera were collected 20 hours after the different immunisations and cytokine levels were measured using the Th1/Th2/Th17 CBA assay. A. Serum IFNα level. B. Serum TNFα level. C. Serum IL-6 level. Bar graphs show cytokine level in pg/ml as mean +/- SEM for 3 samples per group. Data are representative of 3 independent experiments. *, p <0.05; ***, p <0.001.