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Table 1 Improved euthanasia criteria

From: The dark side of ID8-Luc2: pitfalls for luciferase tagged murine models for ovarian cancer

Criteria of euthanasia Liao et al. Improved criteria
Weight Cachexia Loss of 2 g in 48 h
Loss of 3 g in 7 days
Clinical deterioration Increased respiratory frequency Increased respiratory frequency
Anorexia Hunched back with tremor
Ascites /
/ No spontaneous movement when nudged
  1. Weight and clinical status should be evaluated once every 48 h. The improved criteria allow for more objective criteria for euthanasia of animals