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Fig. 2 | Journal for ImmunoTherapy of Cancer

Fig. 2

From: Enhanced recruitment of genetically modified CX3CR1-positive human T cells into Fractalkine/CX3CL1 expressing tumors: importance of the chemokine gradient

Fig. 2

Transduction of the CX3CR1 receptor in human T cells and functional validation. a Population of CD3+ and of CD4+/CD8+ T cells post retroviral transduction of eGFP/CX3CR1 or -eGFP gene after 10 days of in vitro culture with hIL-2 medium. b Representative flow cytometry plot showing the transduction efficiency in eGFP or CX3CR1-eGFP in human T cells. Cells were gated on live cells followed by subsequent gating on CD3, CD4 and CD8 positive T cells. c mRNA expression of CX3CR1 in transduced CD3/IL-2 activated human T cells (Bars, Triplicates +/−SEM *P < 0.05, **P < 0.01 for difference between eGFP and CX3CR1-eGFP-T cells (Student’s t test). d Transwell migration assay of eGFP-T cells or CX3CR1-eGFP T cells in response to different concentrations of rhCX3CL1, ****P < 0.0001, Two-way ANOVA

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