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Fig. 3 | Journal for ImmunoTherapy of Cancer

Fig. 3

From: Enhanced recruitment of genetically modified CX3CR1-positive human T cells into Fractalkine/CX3CL1 expressing tumors: importance of the chemokine gradient

Fig. 3

Adoptive transfer of CX3CR1-positive T cells to mice bearing NCI-H630 tumors. a Schematic representation of adoptive transfer of eGFP or CX3CR1-eGFP expressing T cells in NSG mice implanted with NCI-H630 tumors. b Representative flow cytometry plot of explanted and disaggregated tumors for the analysis of infiltrating human T lymphocytes (CD45 + CD3+/CX3CR1+) after adoptive transfer. Cells were gated on live cells, the plots show the population of CD3/CD45 positive cells and CD45/CX3CR1 positive cells within the tumors. c Proportion of CD8+ and CD4+ subpopulation within CD3 positive TILs. d Proportion of CX3CR1+ T cells in gated CD3+ tumor-infiltrating T cells (blue line: mice receiving CX3CR1-expressing T cells, red line: mice receiving eGFP-T cells CD3). e Summary of percentage of total CD3+ (left) and CX3CR1+ (right) T cells in explanted tumors after adoptive transfer, obtained from different mice (each dot represents a single mouse). +/−SEM **P < 0.01 for difference between control (eGFP) and test (CX3CR1) group (Student’s t test)

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