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Fig. 3 | Journal for ImmunoTherapy of Cancer

Fig. 3

From: Local angiotensin II contributes to tumor resistance to checkpoint immunotherapy

Fig. 3

AngII signaling blockage reverses immunosuppressive tumor microenvironment. a Representative FACS plot of Teff (CD8+CD44+) and Treg (CD4+Foxp3+) in 4T1 breast tumors from BALB/c mice treated with different Ang II-receptor blockers. Bar chart (right) indicated statistic difference (**, P < 0.01; ns, no significance). Data are presented as mean±SEM, n=3. b Bar chart indicated Teff/Treg rate (**, P < 0.01). Data are presented as mean±SEM, n=3. c Immunofluorescence analysis showed lots of α-SMA positive fibroblasts and less CD8-positive T cells in hypoxic regions ( pimonidazole-positive ) of 4T1 tumors. AGT gene silence in 4T1 cells obviously inhibited the infiltration of fibroblasts and increased CD8-positive T cells. There were an accumulation of CD206-positive (a marker of mouse M2-phenotype TAMs) cells in pimonidazole-positive hypoxic regions of 4T1 tumors while AGT gene silence remarkably decreased the infiltration of CD206-positive cells in 4T1 tumors, especially in hypoxic regions. d Candesartan abated collagen fiber deposition in 4T1 tumors as detected by Sirius Red staining. e Representative FACS plot of TAMs (CD45+CD11b+F4/80+CD206+) in 4T1 tumors with or without candesartan treatment. f Representative FACS plot of Mo-MDSCs (CD45+CD11b+Ly6GlowLy6Chigh) and G-MDSCs (CD45+CD11b+Ly6GhighLy6Clow) in 4T1 tumors with or without candesartan treatment. g Bar chart indicated the percentages of TAMs, Mo-MDSCs and G-MDSCs in 4T1 tumors (**, P < 0.01). Data are presented as mean ± SEM, n=3

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