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Fig. 5 | Journal for ImmunoTherapy of Cancer

Fig. 5

From: A novel spontaneous hepatocellular carcinoma mouse model for studying T-cell exhaustion in the tumor microenvironment

Fig. 5

TAA-specific CD8+ T cells were detected in Akt1/N-Ras/Ag-induced HCC-bearing mice, however showed an exhausted-like phenotype in TME. a Gating for flow cytometric analysis of HBs190–197 or HBc93–100-specific CD8+ T cells among tumor-associated CD8+ T cells of mice receiving HDI of pKT2/CLP-AKT-LUC or pKT2/CLP-AKT-Ags-LUC, respectively, together with pT/Caggs-NRASV12 and pCMV(CAT)T7-SB100. The Percentage (top panel) and the absolute cell number (bottom panel) of b HBc93–100-specific CD8+ T cells and c HBs190–197-specific CD8+ T cells in nodular and diffused tumors expressing Ags (Ag+) or not expressing Ags (Ag-). Mean fluorescence intensity (MFI) of expression levels of PD-1, LAG-3, 2B4, and TIGIT on d intra-tumoral HBc93–100-specific CD8+ T cells, HBs190–197-specific CD8+ T cells, intra-tumoral total CD8+ T cells, and splenic total CD8+ T cells and on e intra-tumoral total CD4+ T cells and splenic total CD4+ T cells (n = 3–7 mice per group). *P < 0.05, **P < 0.01 and ***P < 0.001 (unpaired Student’s t-test)

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