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Fig. 5 | Journal for ImmunoTherapy of Cancer

Fig. 5

From: The motility regulator flhDC drives intracellular accumulation and tumor colonization of Salmonella

Fig. 5

Intratumoral flhDC induction increases colonization, dispersion and intracellular accumulation of Salmonella. a) When flhDC was induced in Salmonella, aqueous motility increased by 18% compared to uninducedSalmonella containing the same pBAD-flhDC construct (*, P<0.05). b) Graphical depiction of the dosing scheme. One hour after tumors were placed into devices, Salmonella was administered for 1 hour. Eleven hours after bacterial administration, media with 0.4% (w/v) arabinose was administered to the devices to induce bacterial flhDC expression. c) When F-Sal was administered to devices, bacteria colonies (red arrows) were first detected at 12 hours. At 47 h, colonies formed by F-Sal with intratumorally induced flhDC were larger than control Salmonella (Sal). Scale bar is 100 μm. d) Spatial distribution of intratumoral bacteria. Intratumoral induction of flhDC increased the level of distal bacterial colonization in tumor masses after 47 hours (*, P<0.05). e) Intratumoral induction of flhDC increased overall tumor colonization (*, P<0.05). f) Intratumorally induction of flhDC increased the number of intracellular Salmonella (green). Scale bar is 100 μm. g) Intratumoral flhDC expression increased intracellular accumulation in the distal region (0.6 < x < 1) of tumor masses (*, P < 0.05). h) Induction of flhDC increased intracellular accumulation within entire tumor masses after 36 hours (*, P < 0.05).

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