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Fig. 2 | Journal for ImmunoTherapy of Cancer

Fig. 2

From: The IL-1/IL-1 receptor axis and tumor cell released inflammasome adaptor ASC are key regulators of TSLP secretion by cancer associated fibroblasts in pancreatic cancer

Fig. 2

Treatment of immunodeficient NSG mice orthotopically transplanted with IL-1 positive Hs766T cells plus CAFs with anakinra reduces TSLP expression in vivo. a Treatment schedule. Mice were orthotopically injected on day 0 with Hs766T cells plus CAFs. Hs766T cells alone and CAFs alone were injected as controls. The next day mice transplanted with Hs766T cells plus CAFs started intraperitoneal injection with the two indicated doses of anakinra and mice were sacrificed on day 6 after daily administration. b Hematoxylin and eosin staining of the pancreas. Left, a tumor mass (T) localized within the normal pancreas (N) is evident. Right, at higher magnification, histological features of pancreatic cancer with cancer cells, fibroblasts and extracellular matrix can be recognized. c Human (left) and mouse (right) TSLP mRNA expression in pancreata of NSG mice implanted with CAFs alone (n = 3), Hs766T cells alone (n = 3) or CAFs plus Hs766T cells (n = 3). Significance was determined using Student’s t-test. d TSLP expression in pancreata of NSG mice untreated (NT) (n = 4) or treated with the two indicated doses of Anakinra (n = 4 for each dose). Significance was determined using Student’s t-test. Values significantly different were indicated as: *p < 0.05

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