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Fig. 3 | Journal for ImmunoTherapy of Cancer

Fig. 3

From: The IL-1/IL-1 receptor axis and tumor cell released inflammasome adaptor ASC are key regulators of TSLP secretion by cancer associated fibroblasts in pancreatic cancer

Fig. 3

Tumor cells induce IL-1β secretion in macrophages through the release of alarmins, among which ASC. a IL-1β secretion by THP-1 cells after priming with PMA and stimulation with tumor cell-derived supernatants (PDAC cell sup) (n = 16). LPS was used as positive control. Each symbol refers to a single experiment and represents the mean of duplicate determinations. Significance was determined using Mann Whitney test. b Comparison of cytokine secretion by THP1 treated with PDAC cell sup, as in a. Each symbol refers to a single experiment and represent the mean of duplicate determinations. The values are reported as fold increase relative to those of THP1 stimulated in the presence of PMA only. Significance was determined using Mann Whitney test. c ASC mRNA expression in the indicated PDAC cell lines. d WB analysis of ASC protein in the indicated PDAC cell lines. e WB analysis to determine levels of ASC protein in TCA precipitated supernatants of the indicated PDAC cell lines. f Immunofluorescence analysis of ASC expression in LPS treated THP-1 (positive control) and the indicated PDAC cell lines. ASC aggregates/specks are represented by green dots (arrows)

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