- Research article
- Open Access
Generation of monocyte-derived tumor-associated macrophages using tumor-conditioned media provides a novel method to study tumor-associated macrophages in vitro
© The Author(s). 2019
- Received: 2 November 2018
- Accepted: 16 May 2019
- Published: 28 May 2019
Tumor-associated macrophages (TAM) are expanded and exhibit tumor-promoting properties within the tumor microenvironment. Current methods to study TAM have not been replicated across cancer types and often do not include exogenous growth factors from the tumor, a key factor in TAM differentiation in vivo.
In this study, an in vitro method to generate monocyte- derived TAM using tumor- conditioned media (TCM) and a cytokine cocktail containing IL-4, IL-10, and M-CSF was utilized to study the phenotype, morphology, and function of TAM across multiple cancer types. TCM was generated from two breast cancer cell lines and an Epstein-Barr virus-positive lymphoma cell line. The properties of in vitro generated TAM were compared to in vitro generated M1 and M2- like macrophages and TAM isolated from patients with cancer.
TAM generated in this fashion displayed an increase in CD163/CD206 co-expression compared to M2- like macrophages (87 and 36%, respectively). TAM generated in vitro exhibited increased transcript levels of the functional markers IL-6, IL-10, CCL2, c-Myc, iNOS, and arginase compared to in vitro generated M2-like macrophages. Functionally, in vitro generated TAM inhibited the proliferation of T cells (47% decrease from M1-like macrophages) and the production of IFN-γ by natural killer cells was inhibited (44%) when co-cultured with TAM. Furthermore, in vitro generated TAM secreted soluble factors that promote the growth and survival of tumor cells.
Limited access to patient TAM highlights the need for methods to generate TAM in vitro. Our data confirm that monocyte-derived TAM can be generated reliably using TCM plus the cytokine cocktail of IL-4, IL-10, and M-CSF. Given the ability of TAM to inhibit immune cell function, continued study of methods to deplete or deactivate TAM in the setting of cancer are warranted.
- Tumor-associated macrophages
- In vitro generation
Tumor progression is influenced by growth factors and cytokines released from an intricate network of immune cells in the tumor microenvironment that significantly contribute to tumor progression . For example, myeloid-derived suppressor cells (MDSC), regulatory T cells, and tumor-associated macrophages (TAM) exist in the tumor microenvironment and have been found to promote tumor growth both directly and indirectly [2–4]. Clinically, the presence of TAM has been associated with a poor prognosis in cancer patients, likely due to their ability to enhance tumor cell proliferation, promote metastasis, and stimulate angiogenesis . These factors highlight TAM as a potential therapeutic target in cancer and provide a rationale for exploring the relationship between TAM and malignant cells.
Broadly, macrophages exist in two major phenotypes, labeled as M1 and M2. Although this classification system can assist the researcher in many ways, it is also possible that this approach could lead to over-simplification of the properties of different macrophage populations. Indeed, the M1/M2 schema has seen the development of multiple sub-types. Classically activated M1 macrophages receive stimulation from IFN-γ, granulocyte-macrophage colony-stimulating factor (GM-CSF), and LPS to produce IL-12 and IL-23 and are involved in promoting type 1 T helper cell (Th1) responses to infection . In contrast, M2 macrophages are alternatively activated by cytokines such as macrophage colony-stimulating factor (M-CSF), IL- 4, IL-10, and IL-13 and produce anti-inflammatory cytokines such as IL-10 and TGF-β. M2 macrophages are able to limit tissue damage caused by inflammatory processes and stimulate tissue repair and remodeling. In vitro, M1-like macrophages can be differentiated from monocytes by stimulation with GM-CSF, LPS, and IFN-γ while M2-like macrophages can be differentiated from monocytes using M-CSF and IL-4. TAM are widely considered to be M2-like macrophages in that they can support tumor growth and suppress local immunity. However, recent studies have concluded that TAM are influenced by environmental factors, such as location within the tumor microenvironment and tumor-derived factors [7–9]. Macrophages have high plasticity and respond to their microenvironment. Therefore, we hypothesize that TAM can be generated in vitro using known methods for differentiating monocytes to M2-like macrophages with the addition of tumor- conditioned media from the tumor type of interest.
Monocytes can be functionally reprogrammed to polarized phenotypes by exposure to exogenous growth factors . In the setting of cancer, monocytes can be recruited from circulation and differentiated into macrophages by M-CSF. Once monocytes are recruited to the tumor, factors in the tumor microenvironment rapidly promote their differentiation into TAM. Tumor-derived factors like IL-10 and IL-4 inhibit the production of pro-inflammatory cytokines and chemokines in macrophages and create an immunosuppressive M2-like phenotype resembling TAM . Therefore, these factors (M-CSF, IL-4, and IL-10) were utilized to generate monocyte-derived TAM in vitro. The tumor-derived factors chosen in this study are consistent with factors found in the tumor microenvironment of various malignancies and are modified from the M2 generation protocol published by Rey-Girand et al. This protocol was modified to fit our hypothesis that tumor-derived factors found in media conditioned from tumor cells (tumor-conditioned media) is needed to polarize monocytes to a TAM phenotype. Therefore, a recipe consisting of IL-4, IL-10, M-CSF, and tumor-conditioned media was utilized in this study to generate monocyte-derived TAM in vitro.
Access to patient TAM is limited. Therefore, in vitro differentiation protocols that recapitulate the phenotype and function of TAM are needed to test new therapeutic intervention strategies that specifically target TAM. However, previously published methods of culturing monocytes for TAM polarization have not been tested and compared across multiple cancer types. Here we have investigated a novel method to generate TAM in vitro that is reproducible in multiple types of cancer. Monocytes were differentiated and polarized into TAM using tumor-conditioned media (TCM) from breast cancer or lymphoma tumor cells and a cytokine cocktail consisting of IL-4, IL-10, and M-CSF. We evaluated the phenotype, morphology, and function of TAM generated via this method in vitro in comparison to in vitro classically activated (M1-like) and alternatively activated (M2-like) macrophages, and to TAM isolated from patient tumor samples.
Cytokines employed were recombinant human IL-4 (Shenandoah, 100–09), recombinant human IL-10 (Peprotech, 200–10), recombinant human M-CSF (Shenandoah, 100–03), recombinant human GM-CSF (R&D Systems, 215-GM-010), recombinant human IL-6 (Shenandoah, 100–10), human IFN-γ recombinant protein (ThermoFisher Scientific, RIFNG100), and recombinant human IL-15 (Shenandoah, 100–86).
The SK-BR-3 and MDA-MB 231 human breast adenocarcinoma cell lines were obtained from American Type Culture Collection. The LK 46 lymphoblastoid cell line (LCL) was generated by infecting naïve B cells with the B95.8 strain of Epstein-Barr virus (EBV). Upon establishment of EBV immortalized lymphoblasts, cells were subcloned and LCLs expanded. EBV gene product expression was validated by immunoblot and shown to be consistent with a type III latency profile. Cells were maintained in RPMI 1640 (SK-BR-3 and LK 46) or DMEM (MDA-MB 231) medium containing 10% fetal bovine serum (FBS) and penicillin/streptomycin.
Harvesting tumor-conditioned media
To obtain culture supernatants for the generation of tumor-conditioned media, approximately 2 × 105 cancer cells per 1 mL were grown to 80% confluence and incubated in 0.2% FBS medium for 24 h. After incubation, the TCM was harvested and centrifuged to remove suspended cells. The supernatant was collected and 10% FBS was added to reconstitute the medium.
Isolation of human monocytes, natural killer (NK) cells, and T cells
Human monocytes, NK cells, and T cells were prepared from healthy donor blood (American Red Cross, Columbus, OH) samples by utilizing the Human RosetteSep Monocyte, NK cell, and T cell Enrichment Cocktail negative selections (STEMCELL Technologies, Vancouver, BC, Canada) respectively and according to the manufacturer’s instruction followed by a Ficoll hypaque density gradient centrifugation technique . Briefly, the appropriate RosetteSep Enrichment Cocktail was added to whole blood at a concentration of 50 μL/mL of whole blood. After incubation at room temperature for 20 min, the blood sample was diluted with an equal volume of PBS and mixed gently. The sample was carefully layered on top of the Ficoll density gradient medium and centrifuged with the brake off. Enriched cell populations were removed from the density gradient medium (plasma interface). After washing with PBS, enriched cells were lysed with red blood cell lysis buffer to remove residual red blood cells. Purity greater than 94% for monocytes, NK cells, and T cells was confirmed via flow cytometric analysis according to CD14, CD56, and CD3 surface marker expression respectively. Monocytes were cultured in 10% HAB medium. NK cells were cultured in RPMI-1640 medium supplemented with 10 ng/mL IL-15. T cells were frozen in freezing media (FBS + 10% DMSO) until the generation of macrophages was complete.
Generation of tumor-associated macrophages
Freshly isolated healthy donor human CD14+ monocytes were plated in a 100 × 20 mm cell culture dish (USA Scientific) at a concentration of 1 × 106 cells per 1 mL media. Monocytes were cultured in a 1:1 ratio of 10% HAB medium and TCM plus the addition of IL-4 (1 μg/mL), IL-10 (1 μg/mL), and M-CSF (1 μg/mL). Medium and cytokines were refreshed every other day and cells were harvested on day 7 using a non-enzymatic cell dissociation solution (Sigma Aldrich).
Monocyte differentiation to M1/M2-like macrophages
For M1/M2 monocyte differentiation freshly isolated human CD14+ monocytes were plated in a 100 × 20 mm tissue culture dish (USA Scientific) at a concentration of 1 × 106 cells per 1 mL of 10% HAB medium. For M1-like monocyte differentiation GM-CSF (1 μg/mL) was added to fresh media every other day. On day 6, media was refreshed with GM-CSF (1 μg/mL), IFN-γ (20 ng/mL), and LPS (50 ng/mL) for 24 h. For M2-like monocyte differentiation M-CSF (1 μg/mL) was added to fresh media every other day. On day 6, media was refreshed with M-CSF (1 μg/mL) and IL-4 (1 μg/mL) for 24 h. Both differentiation conditions were harvested on day 7 using a non-enzymatic cell dissociation solution (Sigma Aldrich). This protocol is based on the Rey-Giraud et al. M1/M2 macrophage generation protocol.
Antibodies used for cell labeling of isolated human monocytes, NK cells, T cells, in vitro generated TAM, M1-like, or M2-like macrophages, or single cell suspensions isolated from tumor or normal human tissue samples were as follows: V450 anti-CD14 (BD Biosciences, 560,349), PE anti-CD56 (Beckman Coulter, IM2073U), PE anti-CD3 (Beckman Coulter, IM1282), PE anti-CD19 (BD Biosciences, 555,413), PE-CF594 anti-CD163 (BD Biosciences, 562,670), PE/Cy7 anti-CD45 (BioLegend, 304,016), FITC anti-CX3CR1 (BioLegend, 341,605), PE anti-CD163 (BioLegend, 333,605), APC anti-CD206 (BD Biosciences, 561,783), and PerCP/Cy5.5 anti-PD-L1 (BioLegend, 329,737). Flow cytometry was performed on a LSR II flow cytometer.
Total RNA was extracted using TRizol reagent (Life Technologies). Reverse transcription reactions were performed using 500 ng RNA in a 20 μL reaction with the high-capacity reverse transcription kit (Life Technologies). cDNA was used as a template to measure the expression of human CD206, PD-L1, IL-6, IL-10, CCL2, c-Myc, MMP9, CSF-1R, iNOS, arginase, TNF-α and VEGF genes by quantitative real-time PCR. Human β-actin served as an internal control for each reaction (Life Technologies). Real-time PCR reactions were performed using the ABI PRISM 7900HT fast Real-time PCR system with SYBER Green (Applied Biosystems) and TaqMan (ThermoFisher Scientific) chemistry.
Human tissue processing
Tumor and normal tissues collected from patients with metastatic melanoma were cut into 1–2 mm3 sections and transferred to a GentleMACS C tube containing PBS. The C tube was run on the GentleMACS dissociator. Dissociated cells were passed through a 70 μm cell strainer. Cells were washed with PBS and stained for flow cytometry. Samples were acquired after patient informed consent was obtained under an Institutional Review Board (IRB)-approved protocol for human subject research (IRB protocol 1999C0348).
T cell CFSE assay
M1/M2-like macrophages and MDA-MB 231 conditioned TAM were generated from healthy donor monocytes as described in Materials and Methods. Autologous T cells were isolated from healthy donor blood. T cells were labeled with CFSE (Life Technologies), non-specifically activated with anti-CD3/CD28 beads (Life Technologies), and co-cultured at a 1:1 ratio. After three days T cell proliferation was assessed by flow cytometry.
NK cell co-culture
Monocytes and autologous NK cells were procured from a healthy donor leukopack and M1/M2- like macrophages and MDA-MB 231 conditioned TAM were generated in vitro. NK cells were added to 96 wells coated with polyclonal human IgG and stimulated with IL-12 (20 ng/mL) and IL-18 (50 ng/mL). Immune cells were added at a 1:1 ratio. M1, M2, and TAM cultured alone in the presence of stimulus were utilized as negative controls. After three days of incubation, supernatants were harvested and analyzed for human IFN-γ using a Duoset ELISA (R&D Systems, DY285).
Measurement of supernatant cytokines and chemokines
For quantitative detection of cytokines and chemokines in macrophage and tumor-conditioned media supernatants, an unbiased pairwise screening of 15 antibodies was performed on U-PLEX plates using an electrochemilluminescence method and read on the Meso QuickPlex SQ 120 (Meso Scale Discovery, 1601 Research Boulevard, Rockville, MD ). All samples were run in batches to minimize inter-assay variability, assayed in duplicate, and quantitated using a standard curve.
Statistical significance of differences between groups was analyzed by ANOVA and a two-tailed Student’s t test, and P ≤ 0.05 was considered to be statistically significant.
Generation of tumor-conditioned media and in vitro macrophages
Characterization of in vitro generation methods of tumor-associated macrophages
Next, the in vitro TAM generation method was further characterized. Freshly isolated human monocytes were cultured in six different conditions. Tumor-conditioned media (TCM) only, cytokine only (IL-4, IL-10, and M-CSF), TCM with IL-4 and IL-10, TCM with IL-4 and M-CSF, TCM with IL-10 and M-CSF, and TCM with IL-4, IL-10, and M-CSF (the last condition being the in vitro TAM generation method). Cells were harvested on day 7 and assessed for surface expression of CD14, CD163, and CD206 (Fig. 2B). Although monocytes cultured with TCM, IL-4, and IL-10 expressed high levels of CD163/CD206 co-positive cells, the surface expression of CD14 was comparable to the monocyte control suggesting the phenotype of cells developed under this condition is different than TAM. (Fig. 2B). In conclusion, the differentiation recipe consisting of TCM with the cytokine cocktail (IL-4, IL-10, and M-CSF) is necessary to fully polarize monocytes to a TAM-like phenotype.
Phenotype and functional characterization of in vitro generated TAM
To expand on these results, qRT-PCR was utilized to characterize the effector functions of in vitro generated TAM. Gene expression of IL-6, IL-10, CCL2, c-Myc, iNOS, MMP9, CSF-1R, arginase, TNF-α, and VEGF were evaluated (Fig. 3B and Additional file 1: Figure S1). Gene expression of IL- 6, IL-10, CCL2, c-Myc, iNOS, CSF-1R, and MMP9 was elevated in all three of the in vitro TAM preparations which suggests that in vitro generated TAM are immunosuppressive and thus tumor promoting. However, an increase in TNF- α and VEGF by qRT-PCR was not observed in these TAM (Additional file 1: Figure S2). This is a limitation of this system, but could be explained by a lack of a hypoxic environment. Tumor hypoxia is thought to be important in the activation of TAM and promotes the release of TNF-α and VEGF . Consistent with previous results, TAM generated from MDA-MB 231, SK-BR-3, and LK46 TCM and the cytokines IL-4, IL-10, and M-CSF were morphologically different from M2- like in vitro generated macrophages. Macrophages were round and had a smooth surface, lacking cellular projections, while TAM were more spindle shaped with distinctly elongated cell projections (Fig. 3C).
Phenotype of patient-derived TAM
In vitro generated TAM inhibit T cell and NK cell function
We and others have shown that myeloid cells can impair NK cell expression of IFN-γ and thus impact NK cell function, potentially impairing tumor surveillance . In addition, we have previously reported that normal monocytes can enhance the NK cell cytokine response due to an interaction between the NKG2D activating receptor on NK cells and its ligand MICA on monocytes . Therefore, we investigated the impact of different monocyte-derived macrophage phenotypes, including TAM, on the NK cell IFN-γ response to stimulation. To investigate the effect of immune cells on NK cell IFN-γ production, NK cells stimulated with plate bound IgG, IL- 12, and IL-18 were co-cultured alone, with M1- like macrophages, M2-like macrophages, or in vitro generated TAM at a ratio of 1:1. Indeed, an enhanced NK cell cytokine response was observed when NK cells were co-cultured with M1-like macrophages . However, when NK cells were co-cultured with M2-like macrophages or in vitro generated TAM, NK cell production of IFN-γ was inhibited (Fig. 5D).
In vitro generated TAM secrete growth factors
In vitro generated TAM produce inflammatory cytokines and growth factors that support the survival of tumor cells. We therefore wished to perform a comprehensive analysis of the immunomodulatory factors derived from the TAM being studied in this report. Supernatants from M1 and M2-like macrophages and in vitro generated TAM conditioned with MDA-MB 231, SK- BR-3, and LK 46 TCM were assessed using a U-PLEX -platform designed to assess the profile of soluble factors involved in the pro-tumor function of TAM. Overall, in vitro generated TAM had high expression of several tumor-derived chemoattractants including chemokines CCL2(MCP-1), CCL7(MCP-3), macrophage inflammatory protein (MIP)-1β, and vascular endothelial growth factor (VEGF)-α, which have been shown to promote monocyte recruitment to tumors in experimental mouse models of lung, breast, and pancreatic cancer [30–33] (Fig. 6).
The current study provides evidence of an effective method to generate and study TAM in vitro using TCM and the cytokines IL-4, IL-10, and M-CSF. The results demonstrate that in vitro generated TAM are phenotypically distinct from both M1 and M2- like macrophages and co- express the TAM markers CD163 and CD206. In vitro generated TAM share this phenotype with human TAM isolated from melanoma tumors. Interestingly, our study demonstrated crosstalk between TAM and NK cells and TAM and T cells. NK cells are lymphoid cells that are known to play a key role in cancer immunosurveillance. NK cell function was impaired when NK cells were co-cultured with TAM, an interaction that was characterized by a decrease in NK cell IFN-γ production. These results support the ability of TAM to impair the anti-tumor response of NK cells. While the suppressive factors produced by TAM that contribute to the inhibition of NK cells is not fully understood, it has been reported that expression of PD-L1 (highly expressed on in vitro generated TAM, Fig. 5A) and secretion of TGF-β by TAM are likely involved [23, 25, 28, 29].
In vitro generated TAM also impaired T cell function. The ability of in vitro generated TAM to inhibit CD8+ and CD4+ T cell proliferation suggests that targeting TAM in the setting of cancer could enhance NK and T cell function for improved cancer control. These results support the concept that in vitro generated TAM have the ability to impair the anti- tumor response of NK cells and T cells and suggests that PD- 1/PD-L1 directed therapies could exert a direct effect on macrophages in cancer types with implications for cancer treatment with checkpoint inhibitors.
Although protocols for in vitro differentiation of macrophages have been published, a detailed characterization of TAM generated in cultured tumor conditions has not yet been described. Several groups have reported protocols for generating M1/M2 macrophages that employ stimulation with GM-CSF and/or M-CSF, respectively [34–36]. Rey-Giraud et al. has described the influence of culture conditions on monocyte-derived macrophage phenotype and function. Several groups have reported on monocyte polarization methods that further subdivide M2 macrophages into M2a (cultured with M-CSF + IL-4 or IL-13) and M2c (cultured with M- CSF + IL-10) [37, 38]. This alternative M2 phenotype and function is similar to TAM. Extending these findings, we utilized a culture method that included TCM (media containing factors secreted from tumor cells) in combination with a cytokine cocktail derived from M2a and M2c macrophages including IL-4, IL-10, and M- CSF to develop a method of culturing TAM in vitro specific to the setting of cancer. Characterization of the TCM revealed the presence of several factors (MIP-1β, IFN-γ, and G-CSF) that could explain the phenotype of the in vitro generated TAM. Importantly, this method of TAM generation was reproduced using TCM from three different cell lines, suggesting that this method can be translated across cancer types.
TAM generated in vitro demonstrate an alternative phenotype that correlates with previously published data on M2 macrophages [39–41]. The ability of M-CSF to polarize monocytes to a M2-like phenotype is well documented, however TAM are considered a unique M2-skewed myeloid population which was confirmed in this study . Higher expression of markers for immunosuppression, angiogenesis, and invasion were seen in TAM generated in vitro with TCM compared to M1 and M2-like macrophages. Most notably MCP-1, IL-10, and IL-8 were seen expressed in higher levels by TAM. MCP-1 is one of the most studied factors secreted by tumor cells and macrophages. MCP-1 has been shown to correlate with the number of TAM and with poor prognosis in human and murine tumors [42–44]. In human breast cancer models using MDA- MB 231, MCP-1 recruits monocytes to the tumor, driving growth and metastasis to the lung and bone in nude mice [45, 46]. In vitro generated TAM also produced IL-10, a potent immunosuppressive cytokine that could contribute to T cell regulation [47, 48]. Finally, the proinflammatory cytokine IL-8 has been shown to enhance invasive activity of tumor cells in a human colon cancer cell line . In contrast, the cytokines IFN-γ, IL-6, G-CSF, IL- 13, IL-17A, TNF-α, and IL-1β were expressed at low levels in TAM. Notably, M1-like macrophages expressed the M1 macrophage produced cytokine IL-6, while M2-like macrophages expressed IL-13 [50, 51].
The communication pathways between tumor cells and TAM are intricate and modulation of a single signaling axis to target the immunosuppressive function of TAM may not be effective. The development of future therapeutic strategies to target TAM requires the identification of multiple driving forces in macrophage polarization from the tumor microenvironment. A more comprehensive analysis of TAM in different cancers, cancer stages, and tissue types could provide the basis for a more refined approach to developing TAM targeting strategies. Although this study is limited to the model of two breast cancer and one lymphoma cell line, the system developed in this study can be used with TCM from different cancer types as a first step in this process.
In summary, TAM are involved in the progression of many human cancers. Therefore, it is important to gain a better understanding of how TAM contribute to cancer cell growth and to apply these findings clinically. Recent and ongoing studies targeting TAM demonstrate its promise as a potential cancer therapy. Our data support that in vitro generation of TAM using TCM and the cytokines IL-4, IL-10, and M-CSF provides a novel method to study the phenotype, morphology, and function of human TAM.
This work was supported by National Institutes of Health Grants P01CA95426, K24 CA93670 (W.E. Carson) and T32CA90338–27 (W.E. Carson). The project described was supported by Award Number Grant UL1TR002733 from the National Center for Advancing Translational Sciences.
Study Design: BB, LS, LSK, ES, RB, WEC, TM, KJ. Study Conduct: BB, LS, LSK, MD, AC, ES. Data Collection: BB, LS, LSK, MD, AC, ES, GL, KJ. Data analysis: BB, LS, LSK, AC, ES, TM. Manuscript Write Up: All authors. Final Approval of Manuscript: All authors.
Ethics approval and consent to participate
The study was initiated after approval from the institutional review board (IRB) and following patient signing of informed consent under an IRB approved protocol for human subject research (IRB protocol 1999C0348).
Consent for publication
The authors declare that they have no competing interests.
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